Taking Advantage of Taguchi Design Method to Optimize Medium Culture Conditions for Producing Recombinant Follicle Stimulating Hormone

P-98: The Effect of Primary pH Values of Medium Culture on Expression of Human Follicle Stimulating Hormone in Recombinant Chinese Hamster Ovary Cells.

Background One of the common gonadotropin hormones used for infertility treatment is recombinant Follicle stimulating hormone (rFSH). pH is an environmental factor that can affect production of recombinant proteins with no imposing cost. Culture pH is known as a parameter that significantly affects cell growth and protein production. In this study, tried to investigate the effect of different p...

A cell culture assay for follicle-stimulating hormone.

Cultured rat ovarian granulosa cells respond to follicle-stimulating hormone (FSH) by synthesizing and secreting plasminogen activator. The specificity of the response for FSH prompted us to explore the use of this system as an in vitro bioassay for FSH. The release of FSH by pituitary cell cultures has been examined by this method, as have been preparations of FSH of known biological activity....

Using Taguchi Method to Optimize Recovery of Bismuth by Electrolysis

This paper presents a methodology, based on the taguchi parameter design approach, for the optimization of process parameters for bismuth recovery from aqueous solutions. The process parameters considered are [Bi3+], [NaCl], temperature, and cathodic current density. In addition, cell voltage and current efficiency as two responses have been considered. An orthogonal array L9, the signal-to-noi...

Meta-analysis on recombinant versus urinary follicle stimulating hormone.

A recent meta-analysis (Daya and Gunby, 1999) on recombin-ant versus urinary follicle stimulating hormone (rFSH versus uFSH) deserves methodological comment. The a priori definition of eligibility criteria for studies to be included in a meta-analysis are central and must be systematic. It was up to the authors to decide whether their search would be extended to include all unpublished studies,...

Follicle-stimulating hormone